Electroporation techniques harness precisely controlled electric pulses to transiently disrupt cell membranes, enhancing transport of ions, small molecules and macromolecules. In oncological therapies ...

Researchers have developed a technique that improves and cuts the cost of a technique called electroporation, which involves opening pores in cell membranes using an electric field to introduce ...

Living cells express genes involved in physiological functions like development and metabolism via complex mechanisms. The cell membrane protects the genome from various exogenous molecules. Cell ...

To increase the levels of pulmonary gene transfer by nonviral vectors, we have adopted electroporation protocols for use in the lung. A volume of 100–200 μl of purified plasmid DNA suspended in saline ...

Jackson Laboratory researchers have shown that using an electric current to deliver the CRISPR/Cas9 system, in order to engineer genetic changes in laboratory mice, is highly efficient and ...

Jackson Laboratory researchers have shown that using an electric current to deliver the CRISPR/Cas9 system, in order to engineer genetic changes in laboratory mice, is highly efficient and ...

RNA interference (RNAi), an important tool for gene expression and gene function analyses, can be induced by the introduction of sequence-specific small interfering RNAs (siRNAs) into cells. For an ...

It is well understood that external electric fields can affect the integrity of cell membranes. This is due to an inherent difference between the electric potentials of the interior of a cell membrane ...

Electroporation Improvements, New Reagents, and Easier-to-Use Platforms Impact Bottom Line Transfection technology for inserting nucleic acids into cells is getting a boost from novel electroporation ...

A novel transfection method using water-in-oil droplet electroporation has been developed: a liquid droplet with exogenous DNA and cells that is suspended between electrodes in dielectric oil is ...

Jackson Laboratory researchers have shown that using an electric current to deliver the CRISPR/Cas9 system, in order to engineer genetic changes in laboratory mice, is highly efficient and ...

Modification of cell physiological function with novel parallelized electroporation of mammalian cells by using electrostatic manipulation in a water-in-oil droplet Living cells express genes involved ...