Figure 1: Description and proof of principle for balanced PCR. As an alternative to the separation PCR step, LN1- and LN2-ligated plasmid DNA were mixed in different proportions and used in a ...
The evolution of PCR technology is shaping the future of diagnostics, especially in viral detection and RNA analysis. In the second episode of our New Frontiers in PCR Technology Series, we have ...
A 1.4-Mb tandem duplication, including the gene for peripheral myelin protein 22 (PMP22) in chromosome 17p11.2-12 is responsible for 70% of the cases of the demyelinating type 1 of Charcot-Marie-Tooth ...
Polymerase Chain Reaction (PCR) is a method which utilizes the mechanism of DNA replication to amplify a specific part of DNA. This involves the use of DNA polymerase, which makes new strands of DNA ...
What is real-time quantitative PCR? qPCR uses one of two methods to detect the changes to DNA amplification: use of fluorescent dyes or sequence-specific DNA probes. qPCR is normally performed in a ...
Quantitative polymerase chain reaction, or "qPCR", is considered the “second generation” of PCR and was first described in 1992. The past three decades have seen significant advances in qPCR ...
There are many different technologies available to conduct multiplex PCR. However, each comes with its own sets of pros and cons. This infographic compares three pillars of PCR, quantitative PCR, ...
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