It is the final release of hostages and prisoners in the first phase of the Gaza ceasefire, which began last month.
Amid the most intensive Israeli operations in the area in decades, Palestinians fear entrenched displacement and a return to military control in some towns.
The move came after Israeli Prime Minister Benjamin Netanyahu ordered a stop to the release of Palestinian prisoners and detainees.
The World Health Organization is deeply concerned about violence in the Israeli-occupied West Bank and the impact of what it called "starkly rising" attacks on healthcare, its representative in the Palestinian territories said on Tuesday.
Dozens of released Palestinian prisoners arrived in the West Bank town of Beitunia on Wednesday night and were greeted by a crowd of friends and family members. (AP video shot by Imad Isseid/Produced